微卫星座位数目对家禽遗传距离估测精确度的影响

Effect of the Numbers of Microsatellite Loci on the Accuracy of the Genetic Distance Estimation in Poultry

以实际测定的29个微卫星座位的基因频率为基础,分析了4个中国地方家禽品种在5、10、15、20、25个微卫星座位情况下的遗传距离估测精确度,结果表明:随着微卫星数目的增多,遗传距离估测精确度精度也随着升高。对5个水平上的遗传距离标准差进行t检验,结果表明:25个座位水平对5、10、15、20个座位水平存在显著或极显著差异,初步得出在分析群体间遗传关系时应选用较多的座位。

Based on the allele frequencies of 29 microsatellite loci, the accuracy of pair wise genetic distance among 4 Chinese native chickens was analyzed at 5, 10, 15, 20 and 25 microsatellite loci levels. The results indicated that the accuracy of genetic distance estimation increased along with the numbers of the loci increase. The result of t test was that 25 microsatellite loci had significant difference with 5, 10, 15 and 20 loci levels. It could be concluded that more possible microsatellite loci should be used when the experiment was designed to analyze genetic relationship between different varieties.