烟草种子萌发期间可溶性蛋白质含量与游离氨基酸含量的相关性研究

The Correlation Between the Soluble Protein Content and the Free Amino Acids During Germination of Tobacco Seeds

为了解烟草种子萌发时可溶性蛋白质含量与游离氨基酸含量之间的关系,研究以烤烟K326种子和白肋烟TN86种子为材料,采用紫外分光光度法,探索了烤烟K326种子和白肋烟TN86种子从萌发时第0小时至第336小时共十六个时期的萌发过程中,可溶性蛋白质含量及游离氨基酸含量的变化规律。结果表明:烤烟K326种子可溶性蛋白质含量高峰,在第96小时达最大值,而白肋烟TN86种子可溶性蛋白质含量高峰,则在168小时达最大值;烤烟K326种子和白肋烟TN86种子游离氨基酸含量最大值,均在第336小时;两种类型烟草种子萌发期间种子中可溶性蛋白质含量与游离氨基酸含量之间均呈极显著正相关。

The purpose of the research is to study the relationship between the content of soluble protein and the free amino acids during the germination of tobacco seeds. Using both flue-cured tobacco K326 seed and burley tobacco TN86 seed as the studying materials, the content of soluble protein and the free amino acids in tobacco seed was tested by ultraviolet and visible light spectrophotometer. In this article, when tobacco seeds germinated, the dynamic changes of content of soluble protein and the free amino acids were concerned with the changes of such components at 0h, 2h, 6h, 12h, 18h, 24h, 30h, 36h, 42h, 48h, 60h, 72h, 84h, 96h, 168h, 336h, respectively. The results showed that the contents of the soluble protein in flue-cured tobacco K326 seed reached its maximum value at the 96th hour, while it was at the 168th hour in burley tobacco TN86 seed. The maximum value at the 336th hour so did the contents of the free amino acids in both flue-cured tobacco K326 seed and burley tobacco TN86 seed during the germination of tobacco seeds. The dynamic changes between the contents of the soluble protein and the contents of the free amino acids presented the very notably positive correlation.