Bt与EoNPV混用配比优劣性图谱分析

Analysis on the Optimal Ratio of Bt to EoNPV

苏云金杆菌(Bt)和茶尺蠖核型多角体病毒(EoNPV)等,两类生物源杀虫剂常以复配混用方式应用。由于药效的迟缓,而明显表现出剂量-时间-致死作用复合特征。但目前尚缺乏与之相适应的混用配比优劣判别标准。受叶庆华等地学信息图谱分析和曹进等指纹图谱整体相似性分析理论和方法的启发,用室内人工饲养繁殖茶尺蠖2龄初—中期幼虫生物测定结果作为基础数据,分析研究了Bt与EoNPV混用的剂量-时间-致死作用复合特征,并进行了配比优劣性判别的尝试。首先将生物测定的有关结果输入SPSS统计分析软件包和Excel绘图软件,进行多因素方差分析,同时分别绘出不同药剂处理的,以剂量梯度为横轴,以累加死亡虫数为纵轴的不同观察时段害虫致死过程的曲线组图,简称时段药效信息图;再使用SPSS软件包进行单因素方差分析,提取出用于整体相似性比较的图谱单元Ⅰ和用于细节非相似性比较的图谱单元Ⅱ,且分别量化,由此得到相似值和非相似值,据此又分别算得相似系数和非相似系数;最后综合成一种混用配比优劣性总体判别指标,简称Q值。结果,Bt∶EoNPV为9∶1、7∶3、4∶1和2∶3等4个混配处理的Q值依次为200、100、31.0和23.8,明显标示了其中“9∶1”的Q值最大,被确认为最合理的混配处理。此结果与之前的研究相同。还对该图谱分析方法的可靠性以及应用的可行性等进行了讨论。

Two bio-insecticides, Bacillus thuringiensis （Bt） and Ectropis oblqua Nuclear Polyhedrosis Virus （EoNPV）, are commonly used for control of pests as mixtures. The efficiency against pests has a complex relationship of dosage-time-mortality because of the bio-insecticides＇ chronic lethal effect. However, evaluating ratios of two bio-insecticides had not been investigated. The present investigation studied dosage-time-mortality on the second instars larva of Ectropis oblqua using the theory and method of tupu analysis from Ye et al and fingerprint chromatogram analysis from Cao et al. Analysis of variance of multiple factors to bioassay data of Ectropis oblqua larva was conducted using the SPSS statistical analysis system and Excel drawing software. Multiple curves of the mortality process of the pest or the efficiency at intervals of time at different doses were established. One-way analysis of variance to the data from the curves was conducted by the SPSS statistical analysis system and similarity, and non-similarity were obtained according to tupu Ⅰ, and Ⅱ , then similarity coefficient, and non-similarity coefficient were calculated respectively. Finally, an evaluation index of efficiency of mixed bio-insecticides to the pest was constructed based on two coefficients, i.e., the Q value. The result showed that Q values of four ratios of Bt：EoNPV, 9： 1, 7：3, 4：1 and 2：3, were 200,100,31.0 and 23.8, respectively, indicating that 9 ： 1 was the best ratio with the maximum Q value. The finding was consistent with previous bioassay. Reliability and application of the tupu analysis method were also discussed.