神经生长因子及其受体在人胚胎结肠神经系统中的表达与分布

Expression and distribution of NGF、P75NTR、TrkA in colon enteric nervous system of human embryos

目的:研究人胚胎结肠神经系统的发育过程中神经生长因子(NGF)与其两个受体TrkA、P75NTR在结肠神经系统中的分布及与结肠神经系统发育的关系。方法:采用一抗为PGP9.5、NGF、TrkA、P75NTR的免疫组织化学技术,显示结肠神经系统中的神经元。结果:随着胚胎结肠神经系统的发育,P75NTR表达水平不断下降,而NGF、TrkA表达水平不断上升,P75NTR与NGF、TrkA的比例在不断变化。结论:NGF、TrkA、P75NTR参与了结肠神经系统的发育。

Objective To observe the expression and distribution of nerve growth factor （NGF） ,P75NTB and TrkA in colon enteric nervous system（ENS）of human embryos and their relationships with the development of colon ENS in human embryos. Method The neurons in colon ENS was shown by using NGF, P75NTR and TrkA as first antibodies in immunohistoehemistry. Result With its development, the expression level of P75NTR descend continuously in colon ENS of human embryos, hut the expression level of the NGF and the TrkA rose continuously, the ratios of the P75NTR and the NGF or TrkA are changing continuously. Conclusion The NGF, TrkA, P75 NTR participates the development of the colon nervous enteric system.