中生菌素产生菌的紫外诱变育种

Breeding of Zhongshengmycin-producing Strain by Ultraviolet Mutagenesis

以中生菌素产生菌C5为出发菌株,经过紫外诱变处理,获得突变株UV-69,其摇瓶发酵效价比出发菌株提高30.2%。传代试验表明,该突变株的高产性能遗传特性较稳定。在15t发酵罐上连续5批验证,平均发酵效价比原生产水平提高47.8%。

As an original strain, the Zhongshengmycin-producing strain C5 was treated by Ultraviolet （UV） Mutagenesis and a mutant UV - 69 was obtained. In the shaking flask, the productivity of strain UV - 69 increased by 30.2 96 compared with the parent strain C5. Submerged culture showed that the hereditary character of high productivity of mutant UV - 69 was stable. When applied in a 15-ton fermentation tank, the average productivity of mutant UV - 69 was 47.8 % higher than that of the original one.