蜂胶遗传毒性研究

Study on Genetic Toxicity of Propolic

目的探讨蜂胶的遗传毒性,为其应用提供安全性毒理学评价依据。方法用鼠伤寒沙门细菌营养缺陷型突变株TA97(a)、TA98、TA100和TA102,采用平板掺入法进行Ames试验,将实验分为加和不加代谢激活系统S9 2组平行试验。受试物蜂胶设5个剂量组(0.005、0.025、0.250、1.000、5.000 mg/皿)。应用小鼠骨髓嗜多染红细胞微核试验,检测小鼠骨髓嗜多染红细胞微核率;利用小鼠精子畸形试验,观察不同浓度的蜂胶致小鼠精子畸形的数目。结果在Ames试验中,蜂胶各剂量组引起的回变菌落数未超过对照组自发回变菌落数的1倍以上;小鼠骨髓嗜多染红细胞微核试验显示,蜂胶3个剂量组的微核发生率均在正常范围内,与阴性对照组比较无显著性差异(P>0.05),与阳性对照组比较差异显著(P<0.05);小鼠精子畸形试验可见,蜂胶3个剂量组的精子畸形率均在正常范围内,与阴性对照组比较无显著性差异(P>0.05),与阳性对照组比较差异显著(P<0.05)。结论蜂胶对所试菌株、小鼠体细胞及生殖细胞无诱变性。

Objective To investigate the genetic toxicity of pmpolic so as to assess its safety. Methods Ames Test was conducted using hisfidine requiting Salmonella typhimurium indicator strains TA97 （a）, TA98, TA100 and TA102. The arginine esterase was tested with the plate incorporation assay with and without metabolic activation system S9 mixture at five concentrations, namely, 0.005, 0.025, 0.250, 1.000, 5.000/plate. Cell micmnucleus test of marrow was used. The micmnucleus polychromatic erythrecytes of marrow were observed and measure. Sperm shape abnormality test was per- formed to assess the deform numbers of sperm. Resultes In Ames test, the mutagenicity of pmpolic group was no mere than 2 times that of the control group. Micmnucle rate and deform numbers of sperm in different groups of pmpolic were all negative （compared with negative control P 〉0.05, compared with positive control P 〈0.05）.Conclusion The propolic is not mutagenic to tested strains, somatic cell and male germ cells in mice.