瓦斯爆炸伤对大鼠肺组织核因子_KB及细胞间黏附分子-1表达的影响及意义

Effects and significance of gas explosion on expression of NF-κB and ICAM-1 in lung tissue of rats

目的研究瓦斯爆炸伤肺组织中核因子kB(NF-kB)及细胞间黏附分子-1(ICAM-1)的表达及二者之间关系。方法建立瓦斯爆炸伤大鼠模型,用地高辛标记NF-kB cDNA探针检测肺组织NF-kB mRNA的表达,用免疫组化SP法检测肺组织NF-kB及ICAM-1的表达。结果瓦斯爆炸伤后各实验组大鼠肺组织中NF-kB mRNA及其蛋白表达、ICAM-1的蛋白表达均较对照组明显增多,在伤后2 h增多最明显。肺组织病理学检查显示,瓦斯爆炸伤后,部分气管黏膜上皮脱落、管壁明显充血、水肿、中性粒细胞浸润；肺泡出血、水肿、大量炎细胞浸润。电镜示Ⅱ型肺泡上皮细胞变性脱落,改变明显。结论瓦斯爆炸伤致NF-kB活化,并促进ICAM-1的表达,与急性肺损伤关系密切。

Objective To explore the expression of NF-κB and ICAM-1 in the gas explosion wounded lung of rats and the relationship. Methods Digoxin labeled NF-κB was used as probe. In situ hybridization was performed to detect the NF-κB mRNA. lmmunohistochemistry was used to detect the expression of NF-κB and ICAM-1. Results The levels of NF-κB mRNA, the expression of NF-κB and ICAM-1 in the wounded rats were significantly increased and reached their peak two hours after injury. Pathology of lung tissue showed that some crachea epithelium mucosae were desquamated; congestion, edema of crachea wall and infiltration of neutrophilic granulcytes were found;hemorrhage,edema and infiltration of lots of inflammatory cells were present inalveolus cells. Electron microscope showed that type Ⅰ , especially type Ⅱ alveolus epithilia had degeneration and desquamation. Conclusion The injury of gas explosion can activate NF-κB, which has close correlation with the acute injury to lung.