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用^(32)P后标法检测三氯乙烯所致DNA损伤的研究 被引量：2

Determination of trichloroethylene induced DNA damage with 32P-Postlabeling
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摘要 目的探讨三氯乙烯(TCE)4周静式吸入下对雄性大鼠所致DNA损伤。方法将TCE分为4个浓度组,对雄性大鼠静式吸入染毒,用^(32)后标法检测肝脏组织和外周血白细胞(WBC)DNA损伤。结果与对照组比较,剂量组放射自显影指纹图多2～3个斑点；1～4周各剂量组肝脏组织和外周血WBC TCE-DNA加合物含量增加,与对照组比较,差异均有统计学意义(P＜0．05)；各浓度组TCE- DNA加合物含量均随TCE染毒剂量量的增加而增加,呈一定的线性趋势和剂量-效应关系；经4周染毒后再饲养1周的大鼠肝脏组织和外周血WBC TCE-DNA加合物各浓度组间差异无统计学意义(P＞0．05)；同一剂量组1～4周肝脏组织TCE-DNA加合物含量均先升高后降低再升高,而外周血WBC TCE-DNA加合物含量同一剂量组各时间点差异无统计学意义(P＞0．05)；各剂量组肝脏组织和外周血WBC TCE-DNA加合物相关性良好。结论4周静式吸入TCE对受试动物有一定的DNA损伤。
作者 胡训军 卢伟 王文静 肖萍 陈良
机构地区 复旦大学公共卫生学院劳动卫生教研室 上海市疾病控制中心
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2006年第7期427-429,共3页 Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词 三氯乙烯 DNA损伤 加合物
分类号 R131 [医药卫生—劳动卫生]
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参考文献24

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二级参考文献6

共引文献21

同被引文献21

  • 1徐新云.职业接触三氯乙烯的皮肤损害特点及诊断探讨[J].中国公共卫生,2004,20(2):251-252. 被引量：38
  • 2李戈怡,杜群芳,徐新云,杨荣兴,张志光,钟平.三氯乙烯引起皮肤和肝脏损害的病例报告[J].职业与健康,2006,22(14):1060-1061. 被引量：7
  • 3张耘.三氯乙烯引起急性重度中毒性肝病的病例报告[J].职业与健康,2007,23(8):599-600. 被引量：4
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中华劳动卫生职业病杂志
2006年 第7期

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