HPLC法分离制备斑衣蜡蝉中的吲哚类生物碱

SeparationandPreparationofIndoleAlkaloidsinLycormadelicatulaWhite.byHPLC

采用ＢＩＯ－ＲＡＤＨｉ－ＰｏｒｅＲＰ３１８柱，以８０％甲醇－水（梯度设置）为流动相，检测波长为２５４ｎｍ，首次从传统昆虫类中药樗鸡（学名斑衣蜡蝉）中分离并制备了４种生物碱。经化学和光谱鉴定，确定了其中２种吲哚类生物碱β－ｙｏｈｉｍｂｉｎｅ和ａｊｍａｌｉｃｉｎｅ。

AHPLCmethodforseparatingandpreparingindolealkaloidsisdescribed.HPLCconditionsforana-lysis:BIO-RADseries700HPLC,model700datastation,UV:model1740UV-VISmonitor,column:BIO-RADHi-poreRP318,250mm×10mm,mobilephase:80%methanol-H2O(gradient),flowrate:1.5ml/min,dc-tectionwavelength:254nm.Onthebasisofspectral(1HNMR,13CNMR,H-HCOSY,MS,DEPT)andchemicalevidence,thestructuresoftwocompoundswereelucidatedasβ-yohimbine(yohimban-16-carboxylicacid-17-hy-droxymethylester(3α,16α,17β))andajmalicine(oxayohimban-16-carboxylicacid-16,17-didehydro-19-ethylmethylester(19α)).