烧伤大鼠早期血浆、皮肤创面及脏器TNFα的变化

The changes in TNFα in plasma,organs and eschar of rats after thermal injury

采用35%Ⅲ度烫伤大鼠模型,对烧伤大鼠早期(72小时内)8个时相点的血浆、皮肤创面及5种脏器的 TNFα含量进行了综合性分析与比较,旨在探讨烧伤早期机体上述部位 TNFα变化规律及其影响。结果表明:血浆 TNFα水平伤后均较伤前有显著升高,12小时到达峰值(P<0.01),8、72小时两个时相点升高有显著意义(P<0.05)。皮肤创面 TNFα水平自伤后1至12小时(四个时相点)均出现非常显著升高(P<0.01)。5种脏器的含量,伤后1、3小时无变化(P>0.05),自8至24小时分别出现异常增高,并达到峰值(P<0.01)。提示;①严重烧伤后大鼠出现血浆、皮肤创面及五种脏器 TNFα含量的异常增高,其出现峰值与当今烧伤病理生理变化高峰期相一致(伤后24小时内);②皮肤创面无论伤前或伤后不同时间的均值显著高于血浆及五种脏器的2.75～86.7倍,它的异常增高可能是造成烧伤后机体TNFα异常的主要来源。

The TNFα(Tumor Necrosis Factor-α)contents in the plasma,five organs(heart,lung, liver,kidney and intestine)and burned skin were measured by ELISA method in Wistar rats with 35% TBSA Ⅲ°.The animals were divided into eight different time groups.The experiment showed that TNFα contents were excessively high in early stage of burn(24 hours postburn)in plasma,eschar and the five organs.The contents of TNFα in eschar were 27.5～86.7 times higher than that in plasma and the five organs.