摘要
利用 TBSA20%,Ⅲ度烧伤家兔模型,在排除了麻醉干扰和尽量剔除了动物菌血症及可能的脓毒症影响的条件下,对烧伤血清的免疫抑制性进行了探讨。旨在观察家兔烧伤后血清是否存在免疫抑制性。结果表明:烧伤动物血清仍存在免疫抑制性,且于伤后第4天所获血清的抑制性最强。利用超滤膜分离伤后第4天血清.发现分子量低于1万及1～3万的烧伤血清超滤物免疫抑制性最强,3万以上的烧伤血清超滤物也具有较强的免疫抑制性。提示:烧伤血清免疫抑制物成分较为复杂,分子量范围较宽。尽管在动物烧伤后第4天的血清中未能发现明显的蛋白电泳异常带,但低分子量的烧伤血清物质中可能存在着主要的免疫抑制物。
A burned rabbit's model (20% TBSA) was used in this study.The sera taken from the burned rabbits without baeteremia and sepsis showed immune suppressive effects,especially the sera ob- tained 4 days after injury.After being separated by ultrafiltration,the burned sera were divided into three groups.The ultrafiltrating substances with molecular weight below 10 Kd or between 10 to 30 Kd were found to reduce the production of IL-2 and the lymphocyte blastogensis stimulated by Con A.The sub- stances with molecular weight greater than 30 Kd also showed profound immune suppressive action on lym- phocyte.No obviously abnormal protein was found in the burned serum analysed with SDS eleetropboresis. It is concluded that the immune suppressive substances in the burned serum were characterised by complex- ity in components and a wide range of molecular weight.
基金
国家自然科学基金
