血管生成素样蛋白3在合并糖尿病肝癌中的表达

Expression of angiopoientin-like protein 3 in hepatocellular carcinoma with diabetes mellitus

目的了解血管生成素样蛋白3(ANGPTL3)mRNA在合并糖尿病(DM)肝细胞癌(HCC)和癌周组织的表达,探讨ANGPTL3和血糖代谢的关系。方法采用逆转录-聚合酶链反应(RT-PCR)检测方法,对45例HCC手术标本(其中合并糖尿病10例)的癌和癌周组织中ANGPTL3 mRNA表达水平进行了相对定量分析。结果合并糖尿病患者的HCC肿瘤组织中ANGPTL3 mRNA的表达强度和未合并糖尿病的肿瘤组织表达强度分别为2.01±0.54和1.25±0.35,(P<0.01)。在癌旁组织中,合并糖尿病的和未合并糖尿病的ANGPTL3 mRNA表达强度分别为1.55±0.46和0.95±0.35(P<0.05)。结论ANGPTL3在肝癌患者合并糖尿病中起重要作用。

Objective To study the expression of angiopoientin-like protein 3 （ANGPTL3） mRNA in hepatocellular carcinoma （HCC） patients with diabetes and in the pericancerous tissue and explore the role of ANGPTL3 in glucose metabolism in HCC patients. Methods Surgical samples of 45 patients with HCC and 10 with diabetes were studied using reverse transcription-polymerase chain reaction （RT-PCR）. The relative level of ANGPTL3 mRNA expression was measured by determining a ratio of PCR products of ANGPTL3 to that of β-microprotein gene. Remits Expression of ANGPTL3 mRNA in tumor tissue was found in 2.01±0.54 of patients with diabetes, whereas it was seen in 1.25±0.35 of patients without diabetes. Expression of ANGPTL3 mRNA in the nontumorous liver tissue was found 1.55±0.46 and 0.95±0.35repectively in patients with and without diabetes. Conclusion ANGPTL3 may play an important role in the glucose metabolism in HCC patients with diabetes.