摘要
快速、高效而灵敏的分离技术对于DNA的分析是至关重要的。使用无胶筛分介质的毛细管电泳是最重要的DNA分离技术之一,通常使用无交联的高分子溶液作为无胶筛分介质。本文在介绍高分子溶液理论的基础上,综述了DNA在毛细管电泳无胶筛分介质(缠结溶液和稀溶液)中的分离机理,主要包括Ogston筛分模型、各种修正的爬行模型、瞬态缠结偶合机理及其改进机理等。
Fast, highly efficient, and sensitive separation technique is crucial for DNA analysis. The capillary electrophoresis using non-gel sieving matrices is one of the most important techniques for DNA separation. The uncrosslinked polymer solutions are generally used as sieving matrices. Based on the introduction of the theory of the polymer solutions, the mechanisms of DNA separation by the capillary electrophoresis in non-gel sieving matrices (entangled and dilute solutions) are reviewed in this paper, which includes Ogston sieving model, various modified reptation models, transient entanglement coupling mechanisms and its improved mechanisms.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2006年第7期987-994,共8页
Progress in Chemistry
基金
国家自然科学基金项目(No.50373040)
教育部留学回国人员科研启动基金项目
安徽省人才开发资金项目(No.2005Z026)资助
关键词
毛细管电泳
DNA分离
无胶筛分介质
高分子溶液
机理
capillary electrophoresis
DNA separation
non-gel sieving matrices
polymer solutions
mechanisms