毛细管电泳无胶筛分介质分离DNA的机理

Mechanisms of DNA Separation by Capillary Electrophoresis in Non-Gel Sieving Matrices

快速、高效而灵敏的分离技术对于DNA的分析是至关重要的。使用无胶筛分介质的毛细管电泳是最重要的DNA分离技术之一,通常使用无交联的高分子溶液作为无胶筛分介质。本文在介绍高分子溶液理论的基础上,综述了DNA在毛细管电泳无胶筛分介质(缠结溶液和稀溶液)中的分离机理,主要包括Ogston筛分模型、各种修正的爬行模型、瞬态缠结偶合机理及其改进机理等。

Fast, highly efficient, and sensitive separation technique is crucial for DNA analysis. The capillary electrophoresis using non-gel sieving matrices is one of the most important techniques for DNA separation. The uncrosslinked polymer solutions are generally used as sieving matrices. Based on the introduction of the theory of the polymer solutions, the mechanisms of DNA separation by the capillary electrophoresis in non-gel sieving matrices （entangled and dilute solutions） are reviewed in this paper, which includes Ogston sieving model, various modified reptation models, transient entanglement coupling mechanisms and its improved mechanisms.