应用反相高效液相色谱法测定润肠清颗粒中大黄素和大黄酚的含量

Determination of Content of Emodin,Chrysophanol in Runchangqing Granules with RP-HPLC

目的 应用反相高效液相色谱法测定润肠清颗粒中大黄素和大黄酚的含量。方法采用C18柱，流动相为甲醇-0．1％磷酸（80：20），流速为1．0mL/min，检测波长254nm。结果 大黄素在0．026～0．208μg范围内具有良好线性关系，平均回收率为99．98％，RSD为1．55％（n=5）；大黄酚在0．0495～0．3960μg范围内具有良好线性关系，平均回收率为100．18％，RSD为1．17％（n=5）。结论 该方法操作简便，快速，可作为该药质量控制方法。

Objective To develop an RP- HPLC method for the determination of the content of emodin, ehrysophanol in Runehangqing Gran- ules.Methods The supelcosil C18 column with methanol- 0.1% phosphoric acid （80：20） as the mobile phase was used.The flow rate 1.0 mL/min and the detective wave length was 254 nm. Results The calibration curve was linear in the range of 0. 026-0. 208μg, Tne average recovery and RSD of emodin were 99.98%, and 1.55%（ n = 5） respectively.The calibration curve was linear in the range of 0.0495 - 0. 3960μg, The average recovery and RSD of chrysophanol were 100.18 %, and 1.17 % （ n = 5 ） respectively. Conclusion The method is simple, quick, and could be used to control its quality.