微囊藻毒素的2-甲基-3-甲氧基-4-苯基丁酸检测方法的研究

The Research of Monitoring the Microcystins by MMPB Method

2甲-基-3-甲氧基-4-苯基丁酸(MMPB)法是检测微囊藻毒素(Microcystins,MC)的重要方法之一。本文研究了温度、pH值、反应时间和氧化剂浓度等实验参数的影响,并在优化条件下采用气相色谱法对水中的微囊藻毒素进行检测。实验得到的最佳反应条件为:pH=9,初始反应温度2℃反应1 h,再升温至25℃反应2 h;KMnO4的初始浓度为0.0156 mol/L。本法对水溶液中的微囊藻的检出限为0.0225μg,回收率为93.5%。

MMPB method is one of the most important methods to monitor microcystins. In this study, the influencing factors on the recovery of MMPB, such as initial pH, temperature, reaction time and initial concentration of KMnO4 were investigated. The results revealed that when microcystins were oxidized by 0. 0156 mol/L KMnO4 at 2℃ for 1 hour, and then at 25℃ for 2 hours under initial pH 9, the best recovery of MMPB, 93.55% was gained and the detection limit was 0. 0225 μg.