流动注射化学发光分析法测定人血清中白蛋白的研究

The Determination of Human Serum Albumin by Flow Injection-Chemiluminescence

本文对人血清中白蛋白的鲁米诺-K3Fe（CN）6化学发光反应体系进行了较系统的研究。考察了流速、发光试剂浓度、PH值以及增敏剂等对人血清白蛋白分析检测的影响。在优化的实验条件下，测定人血清中白蛋白的线性范围为1．2×10^-11～4．4×10^-8mol／L，R^2=0．9942；方法的检出限（S／N=3）为1．2×10^-11mol／L；回收率范围为90．75％～103．5％，本法对2．9×10^-8mol／L的标准样品平行重复12次测定，其相对标准偏差为3．5％。

A new CL reaction of HSA-Luminol-K3Fe（CN）6 was studied by flow injection method. The factors of influencing CL intensity and the enhancement of the surfactants were investigated. A new method based on the CL reaction for the determination of HSA has been established by optimizing the experimental conditions. The linear range was 1.2×10^-11-4.4× 10^-8 mol/L,R^2=0. 9942. The detection limit for HSA was 1.2× 10^-11 mol/L（S/N= 3） . The recovery rate was at 100 ± 10%. For a standard sample （c=2.9×10^-8mol/L）,the RSD was lower than 5%（n=12）.