用RRS光谱和“同原射线计量分析法”同时测定D-和L-色氨酸对映异构体

Simultaneous Determination of D-&L-Tryptophan Chiral Enantiomer Complexed with β-Cyclodextrin by Using Resonance Rayleigh Scattering Spectrum and "Measurement Analysis of Two Lines with One Same Point"

基于β-环糊精（β-CD）与D-和L-色氨酸对映体形成包结物的光谱差异，利用共振瑞利散射光谱（RRS）和二级散射光谱（SOS）进行分析，采用双组分信号响应形成的两条同原射线进行计量分析，建立了同时测定β-CD包结的D-和L-色氨酸对映异构体的新方法。RRS和SOS对D-和L-色氨酸对映异构体的β-CD包结物测定的线性范围为0～5×10^-5mol／L，方法的检出限均在3．1×10^-8～2．8×10^-7mol／L范围，相对标准偏差（RSD）在2．1％～3．0％之间。本法与荧光光谱和紫外光谱方法对照，结果令人满意。

A novel method for simultaneous determination of D- ＆ L-tryptophan chiral enantiomer complexed with β-Cyclodextrin has been developed. It is based on the difference of resonance rayleigh scattering spectra （RRS） and second order scattering spectra （SOS） for complexes of them with β-Cyclodextrin,through the measurement analysis of the two working curve with one same point of the double-component responded signal. The detection linear range is 0-5×10^-5 tool/L, and the detection limits are 3.1×10^-8-2.8×10^-7 mol/L,with a RSD of 2.1%-2.9%. The method has been compared with the methods of fluorescence spectrum and ultraviolet spectrum method with satisfactory results.