摘要
许多病毒利用程序性-1核糖体移码作为翻译调节机制进行繁殖。程序性酵母的病毒杀伤系统由L-A病毒和M1卫星病毒组成。M1病毒编码一种分泌型毒素K1,能杀死不含病毒的酵母细胞,但有自身免疫功能。L-A通过一定频率-1移码事件进行繁殖,-1移码效率的改变影响L-A病毒的存活,使M1数量快速下降。因此以程序性-1核糖体移码为靶位,建立以酵母病毒杀伤系统为基础的筛选模型,在抗病毒药物的高通量筛选方面有良好的应用前景。
Many viruses use programmed -1 ribosomal frameshift （-1 PRF） as a mechanism of cellular gene expression to ensure their propagation. The Saccharomyces cerevisiae K1 killer system consists of the killer strain, L-A helper virus and M1 satellite virus. The M1 dsRNA genome encodes a secreted K1 toxin which kills sensitive yeast cells lacking M1 virus, whereas M1 virus-containing cells are immunity to K1 toxin. The L-A virus propagates by -1 PRF. Alteration of -1 PRF efficiency inhibits L-A viral propagation, in turn decreases the number of M1 virus. Therefore, -1 PRF would serve as a target for screening of anti-viral agents based on the yeast killer system. A model for high throughput screening of natural anti-viral agents presents a promising strategy.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2006年第8期449-452,500,共5页
Chinese Journal of Antibiotics
基金
广东省自然科学基金(6040330)。
