潘生丁对实验大鼠肝缺血再灌注损伤的影响

Effect of dipyridamole on hepatic ischemia-reperfusion injury in rats

目的探讨潘生丁对肝缺血再灌注损伤的保护作用。方法建立大鼠局部肝脏缺血再灌注模型。将24只健康雄性Wistar大鼠随机分为假手术组、缺血再灌注组、潘生丁预处理组,观察各组血浆肝酶及透明质酸(HA)水平变化和肝组织中丙二醛(MDA)、内皮素(ET-1)含量,并行肝组织病理形态学检查。结果与缺血肝组织相比,潘生丁预处理组肝酶的漏出、血浆HA水平及肝组织中MDA、ET-1的含量明显降低(P<0.01)。肝组织病理学损伤亦明显减轻。结论潘生丁预处理可明显改善肝微循环,减轻肝缺血再灌注损伤。药物预处理可为临床提供一种安全有效的预处理方法。

Objective To investigate the protective effect of dipyridamole on hepatic ischemia- reperfusion injury in rats. Methods The model of partial hepatic ischemia was established in Wistar rats. Twenty-four male Wistar rats were randomly divided into 3 group：sham-operated group（SO）,ischemia-reperfusion group（IR）and dipyridamole preconditioning group（DP）. The activity of plasma en- zyme and hyaluronic acid（HA）and the concentrations of malondialdehyde（MDA）, endothelin-1 （ET-1） in hepatic tissues were determined respectively. The hepatic pathological changes were also observed. Results Compared with IR group, the activity of plasma enzyme and HA and the concentrations of MDA, ET-1 in hepatic tissue were reduced markedly in DP group（P〈0. 01 ）, and the pathological injury in DP group as also alleviated significantly. Conclusion Dipyridamole can improve hepatic microcirculation and has a protective effect on hepatic ischemia-reperfusion injury. Drug preconditioning can supply clinical treatment as a safe and effective preconditioning method.