电磁脉冲对肿瘤细胞的影响

Effects of Electromagnetic Pulses on Tumor Cells

用电磁脉冲处理S180肉瘤细胞,然后研究细胞膜、线粒体、细胞核、内质网、细胞周期、细胞凋亡率、细胞中的超氧化物歧化酶(SOD)、丙二醛(MDA)的变化.结果发现,细胞膜受到一定强度的电磁脉冲处理时,其通透性障碍受到抑制,细胞膜上出现微孔,细胞膜微绒毛受到损伤.细胞周期发生改变,其中G2期发生显著改变.线粒体发生肿胀,嵴消失,内膜断裂.细胞核出现染色质凝集,内质网肿胀.电磁脉冲处理后细胞的凋亡率提高7.8%,与对照组相比显著提高(P<0.05).通过检测SOD活性和MDA浓度,发现电磁脉冲对细胞内的SOD活性和MDA的浓度没有显著性影响(P>0.05).

S180 sarcoma cells were exposed to electromagnetic pulses, and then the cell membrane, mitochondria, nucleus, endoplasmic reticulum, cell cycle, apoptosis rate, Superoxide Dismutase （SOD） and Maleic Dialdehyde （MDA） was studied. As a result, the obstacle of permeability of cell membrane was inhibited, and pores induced by electromagnetic pulses were discovered in cell membrane. The microvillus of cell membrane was damaged. The cell cycle was changed with G2 periods which changed significantly. Mitochondria were changed with swelling, disappearance of ridge, endomembrane breakage. The chromatin was condensed, and the endoplasmic reticulum was swelling. The apoptosis rate of exposed cells rose by 7.8 %, and it was significantly higher than that in control （ P 〈0.05）. Comparing the viability of SOD and the concentration of MDA, it is shown that the electromagnetic pulses hadn＇ t significantly influenced the SOD and MDA.