草鱼生长激素基因在COS7细胞中转染表达的研究

The Expression of Grass Carp Growth Hormone Gene in Transfected COS7 Cells

应用RT-PCR技术克隆草鱼生长激素(gcGH)的cDNA,将此cDNA定向插入真核表达载体VR1020中,构建成重组真核表达质粒VgcGH.利用脂质体法使质粒VgcGH转染哺乳动物细胞COS7,对转染后的COS7细胞进行RT-PCR、ELISA和免疫荧光检测,分别在转录和翻译水平证实gcGH基因在COS7细胞中得到了持续和正确的转染表达.

The cDNA for grass carp Growth Hormone（gcGH）was amplified by RT-PCR. Inserting these cDNA into eukaryotic expression vector VR1020, new recombinant expression vectors called VgcGH were constructed. Using lipofectin methods, the recombinant expression plasmids VgcGH were transfected into one kind of mammalian cell scalled COS7 cells. RT-PCR, ELISA and Immunofluorescence assay confirmed that gcGH gene had been correctely transcripted and translated in COS7 cells,and the expression of gcGH was durative.