亚精胺对渗透胁迫小麦幼苗生理活性的影响

The Effects of Spermidine Physiological Activities of Wheat Seedlings under Osmotic Stress

研究了渗透胁迫小麦幼苗叶片某些生理参数的变化以及亚精胺(Spd)预处理对其变化的影响.结果表明,由-0.5MPa聚乙二醇(PEG)-6000引起的渗透胁迫明显降低了小麦叶片相对含水量(RWC)、叶绿素a、叶绿素b、水溶性和非水溶性蛋白质含量,Spd预处理显著延缓它们的下降;渗透胁迫过程中,脯氨酸和丙二醛(MDA)含量、电解质渗漏在对照幼苗中明显增加,在Spd预处理幼苗中其增加受到明显的抑制.表明Sp预处理能够缓解渗透胁迫对小麦幼苗造成的伤害,提高小麦抗渗透胁迫的能力.

The effects of osmotic stress on changes of some physiological indexes of wheat seedlings and the influences of spermidine （Spd） pretreatment on changes of corresponding indexes were investigated. Under 0.5 MPa polyethylene glycol （PEG）-6000 induced osmotic stress, relativewater content （RWC）, levels of chl a, chl b, soluble and insoluble proteinsin leaves of wheat seedlings obviously decreased, and preliminary supply of Spdcould distinctly alleviate these declines. Contents of proline and malondialdehyde （MDA）, and electrolyte leakage in control seedlings markedly increased during osmotic stress. Increased data of these parameters in Spd pretreatmeat seedlings were significantly retarded. These results suggested that Spd pretreatment could obviously alleviate the injuries of osmotic stress and enhance wheat seedlings＇ resistances to osmotic stress.