大豆花叶病毒5个分离物的鉴定及外壳蛋白序列分析

IDENTIFICATION AND SEQUENCE ANALYSIS OF COAT PROTEIN GENE OF FIVE SOYBEAN MOSAIC VIRUS ISOLATES

通过生物学纯化与血清学鉴定（ELISA）得到5个大豆花叶病毒（SMV）分离物，利用RT—PCR法扩增其外壳蛋白（CP）基因片段并进行测序，结果表明：5个分离物CP基因全长均为795个核苷酸，编码产生265个氨基酸。同源性分析表明，5个分离物核苷酸序列同源性为91．19，6～97．1％，由此推导的氨基酸序列同源性为98．19，5～99．2％。结合5个分离物在8个大豆品种上的致病性反应得出，5个分离物在8个鉴别寄主上的致病性反应和序列差异的比较表明，分离物在8个鉴别寄主上致病性反应相差越大，其CP基因氨基酸序列差异就越大，由此证实SMV的CP基因与致病性反应之间有一定的联系。

5 SMV isolates were obtained by using biological purification and serology （DAS-ELISA）. The coat protein gene regions were amplified by reverse transcription polymerase chain reaction （RT-PCT）. Sequences analysis showed that CP gene of the each SMV isolates had 795 nucleotides in length, encoding 265 amino acids. Comparison of the sequences of CP genes between different isolates showed they shared 91.1%-97.1% nucleotide acids identities and 98.1%-99.2% amino acids identities with each other. The results also revealed that there was significant correlation between CP genes of SMV and symptom reaction of SMV on differential Hosts.