正交旋转回归试验优化纤维素酶发酵培养基

Optimization of the Cellulase Fermentation Media by the Quadratic Rotary Combination Design

利用旋转回归法研究里氏木霉WX-112发酵生产纤维素酶的两个重要因素:微晶纤维素粉(Avicel)和麸皮对滤纸酶活的影响,并拟合出回归方程。经回归分析表明,培养基中Avicel、麸皮的含量及其配比对滤纸酶活有显著影响。通过岭脊分析寻优得出:Avicel最佳浓度为1.34g/dL、麸皮最佳浓度为3.35 g/dL,在此优化条件下滤纸酶活可达6.51 U/mL。用30 L发酵罐进行放大试验,滤纸酶活可达10.84 U/mL,CMCase达到449.57 U/mL。

With the quadratic rotary design, the two important factors-Avicel and wheat bran, which play an important part in cellulase production by Trichoderma reesei WX-112 were analysed and studied. Regression analysis showed that the contents and the proportion of Avicel and wheat bran in the medium influenced the FPA significantly. Through ridge analysis, the optimum Avicel and wheat bran contents were determined as 1.34 % and 3. 35 % respectively. Under this optimized condition, the FPA reached 6.51 U/mL. 10.84 U/mI of FPA and 449.57 U/mL of CMCase were obtained when the process was scaled up to 30 L fermentor.