饱和流出式固相萃取法测定逍遥丸中芍药苷的含量

Content Determination of Peoniflorin in XiaoYao Pill by Saturated Flow-through SPE-HPLC

目的 采用饱和流出式固相萃取（SPE-HPLC）法制备逍遥丸供试品溶液，用高效液相色谱法测定其中芍药苷的含量。方法 ZorbaxSB-C18柱（4．6mm×250mm，5μm），以乙腈-水（20：80）为流动相，流速为1ml／min。检测波长为230nm。结果 芍药苷的测定在0．1232～1．232μg范围内线性关系良好，r=0．9999，平均回收率为99．16％，RSD为1．6％。结论 该方法简便快速、灵敏度高、有效除去药物基质保护色谱柱，可用于逍遥丸中芍药苷的含量测定。

Objective To develop saturated flow-through SPE-HPLC method for the assay of Peoniflorin in XiaoYao pill. Methods Separation was performed on a ZorbaxSB-C18 column（4.6 mm ×250 mm ,5 μm）. The mobile phase consisted of acetonitrile - water（20： 80,v/v）. The flow rate was 1 ml/min. The UV detection was set at 230 nm. Results Peoniflorin had a good linear relationship in the range of 0. 123 2 ～1. 232 μg,the average recovery of XiaoYao pill was 99.16% and the RSD was 1.6%. Conclusion The method is simple , sensitive and rapid , and reduce the damage of base material to the chromatographic column , it can be applied to the determination of peoniflorin in XiaoYao Pill.