反相高效液相色谱法测定复方大黄颗粒中大黄素和大黄酚的含量

Determination of Emodin and Chrysoplhanol in Compound Rhubard Granula by RP-HPLC

目的 建立反相高效液相色谱（RP-HPLC）法测定复方大黄颗粒中大黄素和大黄酚的含量。方法 Alltima C18色谱柱（5μm，150mm×4．6mm）；柱温：30℃；流动相：甲醇-0．1％磷酸（87：13）；流速：1ml／min；检测波长：254nm。结果 大黄素的线性范围为0．096～0．576μg（r=0．9999），平均回收率为99．29％，RSD为0．63％；大黄酚的线性范围为0．0784～0．4704μg（r=0．9998），平均回收率为100．08％，RSD为1.52％。结论 该方法灵敏度高、操作简便、重现性好、效率高。可作为样品的检测方法。

Objective To develop an RP-HPLC method for determination of emodin and chrysophanol in Compound Rhubard Granula. Methods The samples were determined by RP-HPLC with a Alhima C18 column（5μm,150mm×4.6mm） and UV detector at 254 nm. The mobile phase was methonal： 0.1% H3 PO4 （87： 13） at a flow rate of 1.0 mL/min. The column temperature was 30℃. Results The calibration curve of emodin was linear over the ranges of 0.096μg ～ 0. 576 μg （ r = 0. 999 9 ）, average recovery was 99.29 ,RSD was 0.63% ;The calibration curve of chrysophanol was linear over the ranges of 0. 078 4 ～ 0. 470 4 μg （r =0. 999 8）, average recovery was 100.08%, RSDwas 1.52%. Conclusion This method is simple, reliable, efficient, and it provides a reliable method for determination of emodin and chrysophanol in Compound Rhubard Granula.