银杏叶制剂改善MID痴呆大鼠学习记忆障碍的实验研究

An Eexperimental Study of Preparation of Ginkgo Biloba Leaves on Learning and Memory Disorder in a Multiple Ischemic Dementia Rat Model

目的:探讨银杏叶制剂对多发性梗死性脑痴呆(MID)大鼠学习记忆能力的影响,为临床应用提供实验依据。方法:建立MID动物模型,对其进行游水迷宫、伤害性电刺激试验和海马区乙酰胆碱(Ach)含量测定。结果:用药各组大鼠游迷宫所需时间和伤害性电刺激出错次数显著少于模型组(P<0.01),除第1天记忆出错次数差异无显著性外(P>0.05),银杏叶治疗组在游水迷宫所需时间及出错次数上均明显优于西药治疗组(P<0.01,P<0.05)。MID大鼠海马区Ach含量显著降低(P<0.01),经药物治疗后其含量显著提高(P<0.01),且银杏叶治疗组优于西药组(P<0.01)。结论:银杏叶制剂能有效的改善MID大鼠学习记忆能力,同时明显提高海马区的Ach含量。

Objective To explore the effect of preparation of ginkgo leaf on learning and memory disorder in Multi-infarct dementia rats, and provide experimental data for clinical application. Methods The models of multi-infarct dementia in rats were established,Y-maze test,nocuity electric stimulation test and the contents of acetylcholine（Ach） in hippocampus were measured. Results In each treated group, the time of Y-maze test and the frequency of the fault of nocuity electric stimulation were significantly lower than the model group（P〈0.01）. Except the fault frequency at the first day,the time of Y- maze test and the frequency of the fauh of nocuity electric stimulation in ginkgo leaf preparation group were significantly higher than the western medicine group. Ach decreased significantly in hippocampus of the MID dementia rats（P〈0.01）. Medication significantly up-regulated Ach level, moreover ginkgo leaf preparation group was higher than the western group. Conclusion Ginkgo leaf preparation is effective in improving learning and memory dysfunction in multi-infarct dementiarats,it can also upregulate Ach content in hippocampus.