精制血府胶囊对缺氧缺糖心肌细胞一氧化氮合酶基因表达的影响

Effect of Refineil Xuefu Capsule on mRNA Expression of Nitric Oxide Synthase of Hypoxic Ischemic Myocytes Evaluated by Northern Blotting

应用血清药理学、反转录ＰＣＲ（ＲＴ－ＰＣＲ）、Ｎｏｒｔｈｅｒｎ杂交等方法比较研究了精制血府胶囊、血府逐瘀胶囊、硫氮酮对缺氧缺糖培养心肌细胞一氧化氮合酶（ＮＯＳ）ｍＲＮＡ表达及乳酸脱氢酶（ＬＤＨ－Ｌ）、肌酸激酶（ＣＫ）、谷草转氨酶（ＧＯＴ）释放的影响。结果表明：三种药物均可显著提高缺氧缺糖心肌细胞ＮＯＳｍＲＮＡ的表达（Ｐ＜０．０１），且以精制血府胶囊最显著（Ｐ＜０．０１）。三种药物均可显著降低缺氧缺糖心肌细胞ＬＤＨ－Ｌ、ＣＫ、ＧＯＴ的释放（ｐ＜０．０１）。精制血府胶囊和硫氮 酮抑制ＬＤＨ－Ｌ的释放较血府逐瘀胶囊更为显著（Ｐ＜０．０１）。提示：三种药物均具有明显的心肌细胞保护作用，以精制血府胶囊和硫氮 酮为优，这种保护作用与其刺激心肌细胞生成一氧化氮（ＮＯ）有一定关系。

Comparative study on effect of refined Xuefu capsule,Xuefu Zhuyu capsule and diltiazem on mRNA expression of nitric oxide synthase(NOS) and release of LDH-L,CK,GOT of hapoxic hypoglycemic cultured myocytes were studied by using the serum pharmacological method,reverse transcriptase-polymerase chain reaction (RT-PCR) and Northen blotting.It was shown that the three drugs can reduce release of LDH-L,CK,GOT of myocytes (P<0.01),the effect of refined Xuefu capsule and diltiazem were obvious than that of Xuefu Zhuyu capsule in reducing LDH-L release and NOS mRNA expression (P<0.01),and effect of refined Xuefu capsule was the strongest(P<0.01).These findings indicated that all 3 drugs have significant effect in protecting myocytes which related to their effect on NO production of myocyte particularly the refined Xuefu capsule and diltiazem.