中药热毒清对内毒素性DIC家兔血浆白细胞介素8和一氧化氮水平影响的研究

Effects of Injectio Reduqing on Plasma IL-8 and Nitric oxide Levels in Rabbits with Endotoxin induced Disseminated Intravascular Coagulation

本实验采用内毒素所致家兔弥散性血管内凝血（ＤＩＣ）模型，测定外周血白细胞介素８（ＩＬ－８）、亚硝酸根／硝酸根离子（ＮＯ／ＮＯ）水平，补体Ｃ５ａ活性及中性粒细胞（ＰＭＮ）趋化指数，并观察中药热毒清注射液对上述指标的影响。结果表明：模型组血浆ＩＬ－８、ＮＯ／ＮＯ水平及Ｃ５ａ活性、ＰＭＮ趋化指数显著升高，其升高水平和组织、器官的损害程度相一致。热毒清组及地塞米松组上述指标均显著降低（Ｐ＜０．０１），病理组织学改变亦轻。提示：ＩＬ－８、一氧化氮（ＮＯ）参与ＤＩＣ发病机制，而热毒清抑制炎症反应，防治ＤＩＣ的作用机理部分是通过调控细胞因子网络而实现的。

Experiments were performed for investigating the effects of Injectio Reduqing(RDQ) on plasma interleukin-8(IL-8),NO2/NO3,complement 5 a(C5a)and polymorphonuclear neufrophilic leukocyte(PMN) Chemotaxis Index(CI) in rabbits with endotoxin-induced disseminated intravascular coagulation(DIC).The results showed that plasma IL-8, NO2/NO3, C 5a and CI levels of PMN increased markedly in model group,which were confirmed pathologically with obvious damage of tissues or organs.While in RDQ group the abov-mentioned parameters and damage of tissues or organs were reduced significantly(P<0.01).The results suggested that the IL-8 and NO might be involved in pathogenesis of endotoxin-induced DIC,and RDQ could be used in preventing or treating DIC through mechanism of regulation of cytokines network.