摘要
在建立促肾上腺皮质激素释放激素(CRH)放射免疫分析法的基础上,观察温补肾阳代表药物附子的重要有效成分乌头碱对正常大鼠下丘脑CRH含量的影响。结果表明:正常大鼠在腹腔注射乌头碱(1μg/kg,3μg/kg,10μg/kg)7天后,下丘脑CRH含量呈剂量依赖性增高,免疫组化法亦见:下丘脑室旁核CRH神经细胞及正中隆起神经纤维较对照组明显增多增深。提示温补肾阳药物改善下丘脑-垂体-肾上腺轴的可能机理之一是通过兴奋下丘脑CRH神经细胞。
The effect of aconitine on the content of corticotropin-Releasing-Hormone (CRH) in the hypothalamus of the normal rat injected with aconitine intraperitoneally was studied by means of radioimmunoassay (RIA).Aconitine is the main effective component of Radix Aconiti Carmichaeli Praeparata, the representative traditional Chinese herbal medicine of Warm-Supplementing Kidney-Yang (WSKY). Results showed that the content of hypothalamic CRH menifested a dosede-pendent increase after 7 days intraperitoneal injection of aconitine (1μg/kg, 3μg/kg and 10μg/kg).The CRH neurons in the paraventricular nucleus of hypothalamus and the neural fibers of median eminence also demonstrated increases in number and enrichment in gray tone as observed by immunohistochemical method of ABC. These results suggested that one of the possible mechanism of the WSKY drugs in improving the HPA axis might be exciting the hypothalamic CRH neurons.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1996年第9期544-546,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
卫生部研究基金