摘要
筛选得到一株疫霉菌的拮抗青霉Penicillium striatisporumP.st10。该菌株强烈抑制疫霉菌及核盘菌的菌丝生长。无菌滤液抑制辣椒疫霉孢子囊的形成及孢子囊、游动孢子的萌发,且能杀死菌丝细胞。该菌株在辣椒根际有很强的定殖能力,有机肥对其定殖有增强作用,接种后40d在根际的青霉菌数量,青霉处理为0.76×104cfu/g,而青霉菌肥处理为2.55×104cfu/g。盆栽试验显示:处理7d后,土壤只接种辣椒疫霉对照发病率为93%;同时接种青霉培养滤液和辣椒疫霉处理为16%;接种青霉培养滤液、新鲜菌丝和辣椒疫霉处理为15%。
growth of pathogens Penicillium striatisporum strain P. st10 was showed to be highly antagonistic against mycelial Phytophthora spp. and Sclerotinia sclerotiorum, while not antagonistic to other tested plant in plate tests. Investigation of antagonistic mechanism under light microscope showed that the dilution of filtrate from liquid fermentation of the fungus inhibited pathogen by leading to hyphal abnormality, inhibiting germination of spores, and suppressing formation and germination of sporangium of P capsici. P. striatisporum could colonize in pepper rhizosphere. Organic fertilizer could improve the colonization ability. The rate of pepper blight disease reduced notably when soil was treated with the mycelium of the strain and its filtrate in pot test.
出处
《中国生物防治》
CSCD
北大核心
2006年第3期239-243,共5页
Chinese Journal of Biological Control
基金
科技部863项目(2002AA601012-02)
江苏省自然基金(BK2003118)
江苏省农科院基金(6110546)
关键词
青霉
疫霉
拮抗作用
防效
Penicillium striatisporum
Phytophthora capsici
antagonistic fungi
biocontrol effect