摘要
目的用反相高效液相色谱测定苹果渣中槲皮素的含量。方法采用Symmetry C18色谱柱（5μm，4．6mm×150mm），流动相为甲醇：0．4％磷酸（5：5），流速为1．0mL/min，柱温30％，检测波长为368nm。结果槲皮素在0．008～0．04ug呈良好线性关系（r=0．99904），回收率为98．93％，槲皮素的相对标准偏差为2．72％，最低检测限度为2pg。结论方法快速、简单，灵敏度高，样品测定结果为苹果渣综合利用提供理论依据。
Aim To adopt a RP-HPLC method for the determination of quercetin in apple pomace. Methods The content was determination on a Symmetry C is （5 μm ,4.6 mm × 150 mm）column, using methanol -0.4% phosphorin acid（50： 50） as the mobile phase ,and detected at UV 368nm. The column temperature was 30℃ and the flow rate was 1.0 mL/min. Results The method was proved to be linear in the range of 0. 008 - 0. 004 μg with a regression efficient of 0.999 04. The average recovery was 98.93% with RSD 2.72%. The minimum detection limit was 2pg. Conehmion The method was proved to be quick, simple and highly sensitive. The result of sample determination wtll supply reference for many-sided utilization of apple pomace.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期607-609,共3页
Journal of Northwest University（Natural Science Edition）
基金
陕西省教育厅专项基金资助项目(03JK084)
