清肠宝对溃疡性结肠炎小鼠模型IL-4和IFN-y水平的影响

The Influence of QingchangBao on the Levels of IL-4 and IFN-γ of Mice Model with Ulcerative Colitis

通过溃疡性结肠炎（UC）葡聚糖硫酸钠（DSS）动物模型，研究清肠宝调控UC的效果及对IL-4和IFN-γ水平的影响，探讨清肠宝调控UC的机制。将40只昆明小鼠随机分成标准模型组、正常组、清肠宝组和柳氮磺吡啶（SASP）组，在造模同时分别灌胃。各组于7d后，用酶联免疫吸附法（ELISA）检测血清中IL-4和IFN-γ含量；观察各组小鼠的体重变化、结肠重量和溃疡点数；用光学显微镜观察各组结肠组织的病理变化。结果表明，清肠宝具有调控DSS诱导的小鼠溃疡性结肠炎模型及其Th1和Th2 细胞亚群细胞因子IL-4和IFN-γ的作用。清肠宝具有防止DSS模型结肠重量减轻和减少溃疡形成的作用。

This study investigated the effects and mechanism of Qingchangbo（QCB） on control ulcerative colitis（UC） and influence on the levels of IL-4 § IFN-γ of UC mice model induced by dextran sulfate sodium（DSS）.40 Kunming mice were divided into four groups randomly： normal,DSS, DSS＋QCB and DSS＋ SASP. Same time modeled of animal,perfusion stomach of mice respectively. Parameters of colitis severity recorded in the experiments were changes of body weight,colon weight and numbers of ulcer;the changes of colon tissue were observed by light microscope;the levels of IL-4 and IFN-γ in serum were detected by ELISA. The results show that QCB can regulate and control imbalance of Th1 or Th2 subgroup cell factor IL-4 and IFN-γ in DSS induced ulcerative colitis mice model. It suggests that QCB could prevent colon weight decrease and reduce formation of ulcer.