免疫球蛋白G的免疫共振散射光谱分析

Immune Resonance Scattering Spectral Method for the Determination of Trace IgG

在pH7.0Tris-HCl缓冲溶液中及聚乙二醇-20000存在下，羊抗兔IgG与兔IgG的免疫复合物可聚集形成疏水的免疫复合物微粒，在330，400，520nm处有三个共振散射峰，在470nm有一个同步散射峰。IgG浓度在1．33～133．3 μg·mL^-1范围内与470nnm处的散射强度呈线性。借此用于定量分析血清IgG，结果满意。方法检出限为0．99μg·mL^-1.

In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 μg·mL ^-1. The detection limit is 0.99 μg·mL^-1. The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.