甲基强的松龙对脊髓损伤影响的组织学观察

Histologic Changes of the Effects of Methylprednisolone(MP) on Pressural Injury of Spinal Cord Occurred During a Surgical Operation

目的探讨甲基强的松龙对医源性脊髓压迫损伤的影响。方法取SD大鼠27只,随机分为实验组、对照组、空白组三组。实验组和对照组分别于术前和术后3h给予甲基强的松龙剂量30mg/kg静脉推注。维持剂量5.4mg/(kg·h)。空白组术前3h给予相同剂量的生理盐水。以25g·cm致伤量直接打击脊髓致不完全损伤。分别于术后8h、16h、24h,光镜观察受打击部位组织学变化。结果使用甲基强的松龙较不使用损伤反应轻。打击前使用甲基强的松龙处理组损伤较打击后激素处理组轻。结论术前使用甲基强的松龙对脊髓有明显保护作用。可明显减轻医源性脊髓压迫损伤带来的继发损害。

Objective To investigate the effects of methylprednisolone（MP）on pressural injury of spinal cord occurred during a surgical operation. Methods Twenty-seven SD rats were randomly divided into three groups. Rats in the experimental group were given with MP at a dose of 30 mg/kg intravenously 3 hours before operation and were given with a maintenance dosage of 5.4 mg/kg · h while in the control group, MP was injected into rats 3 hours after operation with the same dosage and same mode as the experimental group. In the blank group ,rats were treated with physiological saline. Incomplete injuries were caused by direct striking against spinal cord with a magnitude of 25 g · cm, Histologic changes of the damaged spinal cord were observed under light microscope on the 8th, 16th, 24th hour respectively after striking. Results Histologic changes of the damaged spinal cord in the rats treated with MP were found to be much less than those in the blank group, and similar results were found in rats of the experimental group compared with those in the control group. Conclusion MP could protect the spinal cord from damages occurred during surgical operations and the effects could be enhanced by a dosage of MP using before operation.