碘酸钾存在下木瓜蛋白酶的平行催化氢波研究与应用

Studies and application of parallel catalytic hydrogen wave of papain in the presence of iodate

在0.4 mol/L NH3-NH4C l(pH 8.31)介质中,木瓜蛋白酶(PAP)产生一个极谱催化氢波,峰电位为-1.87V(υs.SCE)。当有碘酸钾存在时,PAP催化氢波峰电流明显增大,且峰电位不变,产生一平行催化氢波。该平行催化氢波被用于测定碘酸根离子氧化原子态氢的表现反应速率常数及提高PAP的分析灵敏度。

Papain （PAP） yielded a polarographic catalytic hydrogen wave with the peak potential - 1.87V（vs. SCE） in 0. 4 mol/L NH3 -NH4Cl （pH 8. 31 ） buffer solution. When iedate was present,the peak current of the catalytic hydrogen wave increased greatly, and the peak potential remained at original position, producing a parallel catalytic hydrogen wave. The parallel catalytic hydrogen wave was used to obtain the apparent rate constant of the oxidation reaction of atomic hydrogen by iodate ion. Further more, it can improve the analytic sensitivity of PAP.