氟伐他汀对肾小球系膜细胞产生细胞外基质的影响

Effect of fluvastatin on production of extracellular matrix in glomerular mesangial cell

[目的]观察不同葡萄糖浓度环境、氟伐他汀对人系膜细胞表达FN、IV型胶原作用。[方法]培养人肾小球系膜细胞,分为正常葡萄糖组、高葡萄糖组及加氟伐他汀组,培养244、8 h收集细胞上清液,用酶联免疫法检测FN、IV型胶原。[结果]在高糖组24、48 h FN分别是(1.552±0.546)和(2.547 0±0.968)ng/mL,明显高于正常糖组244、8 h,FN:(0.777±0.076)和(1.180±0.091)ng/mL(P<0.01);在高糖组244、8 h IV型胶原分别是(64.577±5.543)和(80.140±6.605)ng/mL,明显高于正常糖组24、48 h IV型胶原:(42.468±5.615)和(46.368±5.714)ng/mL(P<0.01)。氟伐他汀可减少高糖浓度下细胞上清液中FN、Ⅳ型胶原的表达水平。在高糖组与氟伐他汀(1μmol/L,10μmol/L)作用24、48 h FN和IV型胶原分别是(0.955±0.102)(、0.858±0.081)ng/mL,(0.652±0.078)(、0.822±0.257)ng/mL(P<0.01)、(34.908±4.283)、(32.163±2.220)、(28.260±4.287)(、23.233±7.284)ng/mL(P<0.01)。[结论]氟伐他汀可抑制高糖刺激的系膜细胞表达FN、Ⅳ型胶原。

[ Objective] To observe the effect of glucose concentration, fluvastatin on human mesangial cells to produce FN and collagen Ⅳ. [Methods] Human mesangial cells were cultured . Then all cultured cells were divided into normal glucose group,high glucose group and experimental groups . Human mesangial cells were incubated with different glucose （5 or 25 mmol/L） with or without different concentrations of fluvastatin for 48 hours. Type Ⅳcollagen and fibronectin （FN） levels were assessed by ELISA. [Results] Human glomerular mesangial cells in different glucose expressed FN and type Ⅳ collagen. Fluvastatin decreased the level of cell culture supernatant of FN and type Ⅳ collagen in high glucose. The levels of FN in 25 mmol/L glucose at 24 or 48 hours being （ 1.552 ±0.546） and （2.547 ±0.968） ng/mL were significantly higher than those in 5.0 mmol/L glucose being （0.777 ± 0.076） and （ 1. 180 ± 0.091） ng/mL （ P 〈 0.01）. The levels type Ⅳ collagen of in 25 mmol//L glucose at 24 or 48 hours being （64. 577 ± 5.543） ng/mL and （80. 140 ± 6.605） ng/mL were significantly higher than those in 5.0 mmol/L glucose being （42.468 ± 5.616） and （46. 368 ± 5.714） ng/mL （ P 〈 0.01）. The levels of FN and type Ⅳ collagen in 25 mmol/L glucose group at 24 or 48 hours were （0.955 ± 0. 102） or （0. 858 ± 0. 081 ） ng/mL, （0. 652 ± 0. 078） or （0. 822 ± 0. 257） （ P 〈 0.01 ） and （34. 908 ± 4. 283） or （32. 163 ±2. 220） ng/mL, （28.260 ± 4.287） or （23.233 ± 7.284） （ P 〈 0.01） respectively. [Conclusion] Fhvastatin decreased expression of FN and type Ⅳ collagen in cultured mesangial cell by high glucose stimulation.