反相离子对色谱法测定环维黄杨星D含量

IP-HPLC determination of cyclovirobuxium D

目的:建立环维黄杨星 D 的含量测定方法。方法:采用 Apollo C_(18)柱(4.6 mm×250 mm,5μm),流动相为5 mmol·L^(-1)庚烷磺酸钠溶液(内含0.15%的三乙胺,磷酸调节 pH 至3.1)-乙腈(73:27,v/v),流速1.0 mL·min^(-1),检测波长203 nm。结果:环维黄杨星 D 主峰峰形对称,与杂质峰分离良好。在0.12～0.72 mg·mL^(-1)范围内线性关系良好(r=0.9994),平均回收率(n=3)为98.4%～99.0%,RSD 为0.3%～0.9%。结论:本法操作简单、结果准确,具有良好的重复性,可用于环维黄杨星D的含量测定。

Objective：To establish a method using IP-HPLC for the determination of cyclovirobuxium D. Methods： The assay was conducted on a Apollo C18 column（4. 6 mm ×250 mm,5 μm） with 5 mmol· L^-1 solution of sodium heptanesulfonate（ mixed 0. 15% triethylamine and adjusted to pH 3.10 with H3PO4 ） -acetonitrile （73：27, v/v）as mobile phase. Flow rate was 1.0 mL · min^-1. The detection wavelength was set at 203 nm. Results：Cyclovirobuxium D obtained good isolation from other substances. The calibration curve was linear in the range of 0. 12 - 0. 72 mg· mL^-1 of cyclovirobuxium D with correlation coefficient 0. 9994. The average recovery （ n = 3 ） of cyclovirobuxium D was 98. 4% -99. 0% ,RSD was 0. 3% -0. 9%. Conclusion：The method is simple,rapid,and can be used for the determination and quality control of cyclovirobuxium D with satisfactory accuracy and reproducibility.