摘要
目的从千层塔植物提取物中分离制备石杉碱甲单体。方法利用高速逆流色谱技术,通过寻找合适的两相溶剂体系及工艺参数,研究及讨论石杉碱甲分离制备的新方法。结果以n-Hexane/n-BuOH/H2O(4∶1∶5,V/V/V)为两相溶剂体系,在优化的工艺参数条件下,利用高速逆流色谱技术,获得了单体纯度为98.6%(HPLC)的石杉碱甲单体。结论利用高速逆流色谱技术成功地从千层塔植物提取物中分离制备了纯度高于98%的石杉碱甲单体。
OBJECTIVE Prepare Huperzine A from herb extract of Huperzia serrata Thunb. （Trev. ）. METHODS Using the technology of HSCCC, research and discuss a new preparative method of Huperzine A by searching a suitable two-phase solvent system and relative technical parameters. RESULTS Using n-Hexane/n-BuOH/H2O （4： 1：5, V/V/V） as the two - phase solvent system, Huperzine A with the purity of 98.6% （HPLC） was successfully obtained under the optimized HSCCC conditions. CONCLUSION HSCCC was successfully used for the preparation of Huperzine A from Huperzia serrata Thunb. （Trev. ） at over 98% purity.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第4期295-297,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
高速逆流色谱(HSCCC)
石杉碱甲
千层塔(蛇足石杉)
植物单体制备
High-speed Countereurrent Chromatography （ HSCCC ）
Huperzine A
Qianceng Ta （ Huperzia serrata Thunb. （Trev.）
Prepare monomer from Natural Herb
