离子对色谱法同时测定万古霉素、去甲万古霉素血药浓度

Determination of blood concentration of vancomycin and norvancomycin by ion-paired HPLC

目的测定盐酸万古霉素，盐酸去甲万古霉素血药浓度。方法采用离子对高效液相色谱法。色谱柱为Shimpack C18柱（150×6mm，5μm），加AlltechC18保护柱；流动相为乙腈-离子对缓冲液（115：885，pH3．1）；流速1mL·min^-1；检测波长236nm，柱温40℃。结果盐酸去甲万古霉素在0．80～80．19μg·mL^-1；盐酸万古霉素在1．08～108．00μg·mL^-1浓度范围内呈良好的线性关系，两者低、中、高回收率在97％～110％之间，RSD在2％～6％之间。结论本方法简便可行，快速可靠。适合于临床血药浓度监测。

OBJECTIVE To determine the blood concentration of vancomycin and norvancomycin. METHODS HPLC was used. CH3CN-ion paired buffer（ 115：885, pH3.1 ） was used as mobile phase on Shimpack C18 （150 × 6mm,5 μm）with column temperature at 40℃. The flow rate was 1mL· min^-1. Detection wavelength was 236nm. RESULTS The calibration curve was linear within the range of 0.80 - 80. 19μg · mL^-1 for norvancomycin and 1.08 - 108.00μg · mL^-1 for vancomycin. The average recovery within high and low blood concentration of norvancomycin and vancmyein were 97% - 110% with RSD among 2% - 6%. CONCLUSION This method was simple,rapid and suitable for the clinical monitor of norvancomycin and vancomycin.