SPME-HPLC法测定水中的酚类化合物

Determination of Phenols in Water by Solid Phase Microextraction with High Performance Liquid Chromatography

采用固相微萃取（SPME）高效液相色谱法（HPLC）同时测定了水中苯酚、4-硝基酚、3-甲基酚、2，4-二氯酚、2，4，6-三氯酚、五氯酚等六种酚类化合物的含量。采用ZORBOXSB—C18柱，以甲醇-1％乙酸水溶液为流动相进行梯度洗脱，流速为1．0mL／min。紫外检测波长为254、280nm。六种酚类化合物的检出限为0．31～1．90μg／L，加标回收率为88％～103％。该方法操作简单．能快竦、缝确她枪测水巾的酚娄化合物．

A solid phase microextraction with high performance liquid chromatography （SPME - HPLC） method was used for the simultaneous determination of six phenols in water, including phenol, 4- nitrophenol, 3-methylphenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol. The chromatographic analysis was carried out using a ZORBOX SB-C18 column. Gradient elution was adopted with the mobile phase consisting of methanol and 1% acetic acid solution. The flow rate is 1.0 mL/min and the wavelengths of UV detector are 254 nm and 280 nm. The detection limits of the six phenols are 0.31 - 1.90 μg/L. The recoveries of the standard addition tests for the six phenols are 88% - 103%. The method is simple in operation and can detect the phenols in water rapidly and accurately.