摘要
目的:比较小鼠免疫共刺激信号B7-1和B7-2转化小鼠淋巴瘤细胞株EL4后,转化细胞介导的抗肿瘤免疫作用。方法:构建逆转录病毒表达载体pLXSN-mB7-1和pLXSN-mB7-2,转染野生型EL4细胞,获得高表达B7-1和B7-2分子的EL4/mB7-1和EL4/mB7-2细胞,并制备成瘤苗;分别应用LDH释放法、MTT法以及ELISA法检测比较两种瘤苗激活的T细胞的杀伤活性、细胞增殖和IL-2的分泌情况。结果:①EL4/mB7-1和EL4/mB7-2瘤苗刺激小鼠脾脏T淋巴细胞分泌IL-2的量明显超过野生型EL-4细胞(P<0.01);②24 h,EL4/mB7-1瘤苗刺激小鼠脾脏T淋巴细胞分泌的IL-2的量超过EL4/mB7-2瘤苗(P<0.05);48 h,EL4/mB7-2瘤苗刺激小鼠脾脏T淋巴细胞分泌IL-2的量超过EL4/mB7-1瘤苗(P<0.05);③EL4/mB7-1瘤苗和EL4/mB7-2瘤苗刺激小鼠T淋巴细胞杀伤肿瘤细胞的活性明显增强。EL4/mB7-2瘤苗刺激小鼠T淋巴细胞杀伤肿瘤细胞的活性与EL4/mB7-1瘤苗相当(P>0.05)。结论:mB7-1和mB7-2分子可活化T细胞分泌IL-2,两者刺激T细胞产生IL-2的高峰时间不同。mB7-2刺激T细胞产生CTL的杀伤活性与mB7-1无明显差别。
Objective： To assess and compare the potential of mouse immune-costimulating signal B7-1 and B7-2 vaccine in inducing anti-tumor immunity effect. Methods： Reverse transcription expressing vector pLXSN- mB7-1 and pLXSN-mB7-2 were constructed to transfect wild EIA cells, then mB7-1 gene and mB7-2 gene were introduced into EIA cells, the positive clone （EIA/mB7-1 and EIA/mB7-2） highly expressing mBT-1 and mB7-2 molecular was selected. In vitro, The secretion of IL-2 of EIA/mB7-1 and EIA/mB7-2 vaccine stimulated-lymphocytes, and the T cell functions of proliferation and cytotoxicity were detected by the method of LDH-release assay, MTY assay and ELISA. Results：① IL-2 production stimulated by EIA/mB7-1 and EIA/mB7-2 vaccine was much higher than wild EIA ceils（ P 〈 0.01 ） ; ② IL-2 production stimulated by EIA/mB7-1 vaccine was higher than EIA/mB7-2 vaccine at 24 hours （ P 〈 0.05）, whereas IL-2 production stimulated by EIA/mB7-2 vaccine was higher than EIA/mB7-1 vaccine at 48 hours （P 〈 0.05）; ③ In vitro, both mB7-1 vaccine-activated CTL and mB7-2 vaccine-activated CTL exhibited a specific cytotoxicity. These two kinds of activated CTL had no significant difference in killing tumor cells （ P 〉0.05）. Conclusion： Both mB7-1 and mB7-2 can activate T cell to produce IL-2, which get to the peak not at the same time. mB7-1- and mB7-2-activated CTL exhibit the similar specific cytotoxicity.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第8期789-793,共5页
Journal of Shandong University:Health Sciences
基金
山东省优秀中青年科学家奖励基金资助课题(02BS095)
