促甲基化因子对西索米星发酵的影响

Effect of Methylation Factors on Sisomicin Fermentation

研究发现，发酵培养基中添加2．0—3．0g,／L蛋氨酸或7．5—10．0mg,／L氯化钴可明显促进西索米星的合成。蛋氨酸的添加时机和添加方式对西索米星产物合成的作用明显不同。在产物合成中前期（30-48h）添加蛋氨酸的效果最佳。当发酵液中蛋氨酸初始浓度为0．656g／L时，与在产物合成初期一次性添加相比，1．5g／L蛋氨酸在产物合成初期、中期和后期均分成3次添加的效果更优，当发酵至91h结束时，发酵液中西索米星浓度可达0．70g／L。

It was found that the supplementation with some components in medium was significant to sisomicin production. Sisomicin biosynthesis was stimulated by 2. 0 - 3.0 g/L methionine or 7. 5 - 10. 0 mg/L cobaltous chloride. The effect of different supplementation time and mode of methionine on sisomicin biosynthesis was obvious dissimilarity. The best supplementation time of methionine was in the former to middle stage （ 30 - 48 h） of sisomicin biosynthesis. While 0. 656 g/L of initial methionine concentration in the ferment broth, compared with one-off supplementation of 1.5 g/L methionine in the former stage of sisomicin biosynthesis, the effect of supplementing 0. 5 g/L methionine each time in the former, middle and latter stage on sisomicin fermentation was better, sisomicin concentration would reach 0. 70 g/L in 91 h.