染色体2p22的过度扩增及hmsh2基因的高表达与卵巢癌紫杉醇耐药相关性的研究

Study on the Relationship of Taxol-resistance of Ovarian Carcinoma with the Amplification of 2p22 on Chromosome and the Expression of hmsh2 Gene

目的研究染色体特定区域DNA拷贝改变、基因差异表达及其与紫杉醇耐药的关系。方法运用比较基因组杂交(comparativegenomichybridization,CGH)及RTPCR技术,分析卵巢癌紫杉醇耐药株染色体基因组变化和hmsh2基因在卵巢癌组织中的表达。结果CGH结果显示最有意义的变化是卵巢癌紫杉醇耐药株OC3/Tax300细胞染色体2p22过度扩增。RTPCR检测发现hmsh2基因在OC3/Tax300细胞高度表达。hmsh2基因表达阳性率在组1和组2分别为93.9%(31/33)及47.6%(10/21),差异有显著性;在低分化癌为93.3%,显著高于中、高分化癌的54.2%。结论2p22拷贝数的过度扩增及hmsh2基因的高度表达可能与卵巢癌紫杉醇耐药相关。

Objective To investigate the difference of DNA copy number and gene expression on chromosome and their correlation with taxol-resistance of ovarian carcinoma. Methods Comparative genomic hybridization and RT-PCR technique were performed on the taxol-resistant cell lines and ovarian cancer tissues to compare the genomic alteration of chromosome and the hmsh2 gene expression. Results CGH results showed that the most interesting finding was the amplification of 2p22 in OC3/Tax300. Over-expression of hmsh2 gene in OC3/Tax300 was detected. The positive expression rates of brash2 had significant difference between group 1 （93.9% ,31/33） and group 2 （47. 6%, 10/21 ）. The positive rate in poor differential cancer was 93. 3 %, higher than that of 54. 2 % in moderate and well differential cancers significantly. Conclusion Amplification of 2p22 on chromosome and over-expression of hmsh2 gene may be associated with taxol-resistance of ovarian carcinoma.