HPV16 E7和Brn-3a在宫颈癌前病变中的表达及其意义

Significance of HPV16 E7 and Brn-3a Expression in Cervical Neoplasia

目的研究HPV16E7和Brn3a在各级宫颈癌前病变(CIN)中的表达情况以及两者之间的关系,从分子生物学水平探讨其作为宫颈癌前病变的特异性标志物的可能性,以期寻找新的宫颈癌前病变筛查方法。方法对71例患者行宫颈薄层细胞学检查(ThinprepCellTest,TCT),并在阴道镜下组织学活检,其TCT残留标本,用RTPCR法检测各标本中HPV16E7和Brn3a的表达。结果HPV16E7和Brn3a表达按细胞学分级和病理学分级其阳性率呈逐级增高趋势,差异均有显著性(P<0.0001)。两者作为宫颈癌前病变和HPV活化状态的诊断标志物,其结果是一致的(P<0.05)。结论TCT残留标本适合进行RTPCR分析研究。HPV16E7可作为宫颈癌前病变和HPV活化状态的生物标志物。Brn3a可作为可靠的CIN3生物标志物和HPV致癌基因转录活化的标志物。HPV16E7和Brn3a相互作用,可能为宫颈高度癌变发展的原因之一。

Objective To examine the potential of HPV16 E7 and Brn-3a as a biomarker for cervical premalignant lesions in ThinPrep smears, and search for the identification of specific biomarkers for dysplastic epithelial cells of the cervix as a new examine ways. Methods Random sample 71 patients to be examined tissue test under the colpornicroscope. RT-PCR was used to detect the mRNA of HPV16 E7 and Brn-3a in 71 ThinPrep liquid-based smears specimens. Results In cytologic and pathologic diagnose grades; the positive rates of HPV16 E7 and Brn-3a are heightened gradually. And there are markerable difference among them（P〈0. 0001）. As the biomarkers of cervical intraepithelial neoplasia and activation of HPV, the results of HPV16 E7 are consistent with that of Brn-3a. Conclusion ThinPrep smears adapt to do RT-PCR. HPV16 E7 should be one of useful biomarkers to indicate cervical intraepithelial neoplasia, which should show the activation of HPV directly. As one of genes joins the process of cervical malignant,Brn-3a should be one another of credibility biornarker and a marker of HPV oncogene＇s activation. The reciprocity of Brn-3a and HPV16 E7 should be a possible reason for the development of high grade cervical premalignant lesion.