高效液相色谱法测定阿奇霉素胶囊含量

Determination of azithromycin capsules by HPLC

目的建立高效液相色谱法测定阿奇霉素胶囊含量。方法色谱柱为Hypersil ODS2（4.6×250mm）；流动相为0．067mol·L^-1磷酸二氢钾溶液（用磷酸调pH为3．5）-乙腈（70：30）；流速为1.0ml·min^-1；柱温：50℃；检测波长：210nm。结果阿奇霉素在进样量2．5～50μg的范围内与峰面积呈良好线性关系（r=0.9999），平均回收率为100．38％，RSD为0．91％。结论本法简便快捷，结果准确，重现性好，可用于阿奇霉素胶囊的含量测定。

Objective To establish a high-performance liquid chromatograph （HPLC） method for the determination of azithromycin capsules. Methods The column was Hypersil ODS2 （4.6× 250 mm）. The mobile phase composed of 0. 067mol· L^-1 KH2PO4 （adjust pH 3.5 with phosphoric acid） -acetonitrile （70 ： 30）. The flow rate was 1.0 ml ·min^-1, and column temperature was 50℃. The detection was 210 nm. Results There was a good linear relationship between azithromycin and peak area when the sample injection was from 2.5μg-50μg （r=0. 9999）. The average recovery was 100. 38% and RSD was 0. 91%. Conclusion This method is simple and believable. It can be effectively used for the quality control of azithromycin capsules.