高效液相色谱法测定宽中老蔻丸中大黄酚的含量

Determination of Chrysophamol in Kuanzhong Laokou Wan by HPLC

目的 建立测定宽中老蔻丸中大黄酚的含量测定方法。方法 高效液相色谱法。用十八烷基硅烷键合硅胶为填充剂，Kmmasil C18柱（4．6mm×200mm）；甲醇-水-磷酸（86：14：0．02）为流动相，流速：1．0mL／min；检测波长：254nm。结果 HPLC法测定大黄酚可达基线分离，进样量在0．0170—0．5448μg时线性良好，得回归方程为Y=74651．99X-186．40．r=0．9999。平均加样回收率为98．36％，RSD为0．51％（n=5）。结论 本方法简便、准确、重现性好，可用于宽中老蔻丸中大黄酚的含量测定。

Aim To establish an HPLC method for determination of Chrysophanol in Kuanzhong Laokou Wan. Method The HPLC method was used for Kromasil C18 column（4.6mm × 200mm） with methanol -water -0. 1% phosphoric acid （86： 14： 0.02） as mobile phase. The flow rate was at 1.0ml/min and the detection wavelength was at 254nm. Results The calibration curve was linear,ranging from of 0. 017 0 to 0.544 8μg （ r =0.999 9）. The average recovery of the method was 98.36%, and RSD was 0.51%. （ n = 5 ）. Conclusion This method was reliable, accurate, and applicable to the quality control of this preparation.