脑损伤急性期脑微血管5-羟色胺、多巴胺含量变化与外伤性脑水肿的关系

Relationship between Changes of 5 hydroxytryptamine and Dopamine in the Brain Microvessels and Traumatic Brain Edema at the Acute Stage of Brain Injury

目的：探讨脑损伤急性期脑微血管中５－羟色胺（５－ＨＴ）、多巴胺（ＤＡ）的含量变化及其与外伤性脑水肿的关系。方法：采用高效液相色谱—电化学检测器，直接检测大鼠脑损伤急性期脑微血管中５－ＨＴ、ＤＡ的含量。结果：脑损伤后０．５小时脑微血管中５－ＨＴ、ＤＡ含量明显增加，伤后６小时仍明显高于对照组，相差非常显著（Ｐ＜０．０１）；同时限检测脑组织水含量明显增高。结论：脑损伤急性期脑微血管中５－ＨＴ、ＤＡ含量明显增高，严重影响脑微血管舒缩功能，损害血脑屏障，可能是外伤性脑水肿发生与发展的重要因素之一。

Aim: To explore the relationship between changes of 5 hydroxytryptamine (5 HT) and dopamine (DA) in the brain microvessels and traumatic brain edema at the acute stage of the rat brain injury. Methods: Both 5 HT and DA levels in the brain microvessels at the acute stage of the rat brain injury were measured by high performance liquid chromatography with electrochemical detector. Results: The levels of 5 HT and DA in the brain microvessels increased apparently at 0.5h, and still higher than control value at 6h after brain injury ( P <0.01). The brain water content increased remarkably in the same time. Conclusion: The increases of 5 HT and DA in the brain microvessels might be one of the important factors for the production and the development of traumatic brain edema following brain injury.