摘要
本研究通过N+注入芽孢杆菌选育高产抗菌物质菌株。注入N+的能量为25keV,剂量为50×2.6×1013、80×2.6×1013、100×2.6×1013、120×2.6×1013和150×2.6×1013N+/m2。结果表明最佳的注入剂量为50×2.6×1013N+/m2,选育出了1株高产菌株(Bacillus subtilisfmbJ224),其抗菌物质产量是出发菌株的1.96倍,经传代培养,其高产性能稳定。同时通过对该菌株的发酵特性研究,发现其产抗菌物质的模式为延滞合成型。
N^＋ ion implantation was used to obtain higher-yield antimicrobial substance. Bacillus subtilis fmbJ was mutated by 25keV N^＋ ion implantation with the dose of 50 × 2.6 × 10^13, 80 ×2.6 × 10^13, 100 × 2.6 × 10^13, 120 × 2.6 × 10^13 and 150 × 2.6 × 10^13 N^＋/m^2. Results showed that the optimal N^＋ ion dose was 50 ×2.6 × 10^13 N^＋/m^2, and a strain of high-yield antimicrobials was obtained and named as Bacillus subtilis fmbJ224. Its antimicrobial substance yield was increased by 96 % than the initial. The fermentation characteristic of the strain was studied, and the mode of producing antimicrobial substance for the selected strain was arrearage synthesis type.
出处
《核农学报》
CAS
CSCD
北大核心
2006年第4期296-298,330,共4页
Journal of Nuclear Agricultural Sciences
基金
江苏省高新技术资助项目(BG2003311)
关键词
N^+离子
芽孢杆菌
抗菌物质
突变体
N^＋ ion implantation
Bacillus subtilis
antimicrobial substance
mutant
