重组PAI－1在大肠杆菌中表达条件的研究及其对t－PA，u－PA抑制的分析

Study of Expression Conditions of Recombinant PAI 1 in E. coli and the Analysis of PAI 1 Inhibition Towards t PA, u PA

带有表达质粒ｐＢＶ２２０／ＰＡＩ－１的大肠杆菌中，在菌体密度为Ａ６００ｎｍ＝０．３～０．４时诱导，表达重组ＰＡＩ－１（ｒＰＡＩ－１）的效率最高。在诱导后的６ｈ以内，ｒＰＡＩ－１的百分含量持续升高。ｒＰＡＩ－１经分离纯化并用盐酸胍激活后，能够与ｔ－ＰＡ及ｕ－ＰＡ反应。

Bacteria of Escherichia coli harboring the expression plasmid, pBV220/PAI 1, might express recombinant PAI 1(rPAI 1) at the highest effeciency when the bacteria were induced at the bacterial density of A 600nm =0.3￣0.4. Furthermore, the percentage of rPAI 1 in the bacteria kept rising within the 6 hours after the induction. The expressed rPAI 1 activated by guanidine HCl could form complexes with t PA or u PA, which might be visualized by SDS PAGE or fibrin autography.